Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative nucleic acidMonkeypox Virus mu seramu yaumunthu, zitsanzo za lesion exudate ndizitsanzo za nkhanambo.Ma seti oyambira ndi ma probe olembedwa ndi FAM adapangidwakuti adziwe zenizeni za Monkeypox Virus, Human RNase P jiniyotengedwa nthawi yomweyo ndi mayeso chitsanzo amapereka mkatikuwongolera kutsimikizira ndondomeko ya nucleic m'zigawo ndi reagentumphumphu.Kafukufuku wolunjika ku jini ya RNase P yamunthu imalembedwa ndi VIC.
| Zigawo | 48 Mayeso / zida | 96 Mayeso / zida |
| PCR reaction buffer | 672 μL × 1 chubu | 672 μL × 2 chubu |
| PCR enzyme mix | 50 μL × 1 chubu | 100 μL × 1 chubu |
| Kulamulira kwabwino | 100 μL × 1 chubu | 200 μL × 1 chubu |
| Kuwongolera kolakwika | 100 μL × 1 chubu | 200 μL × 1 chubu |
1. Kumverera: 200 makope/mL.
2. Mwatsatanetsatane: Palibe mtanda anachita ndi Enterovirus(EV), chikukuvirus(MV), Rubella virus(RV), Varicella-zoster virus(VZV),Dengue virus (DenV), HumanParvovirus B19 (HPVB19),Epstein-barr virus (EBv), human herpes virus 6 (HHV-6)
3. Kulondola: CV ≤ 5%.
Real-Time PCR System: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-Gene TM 6000/Q, Bio-Rad CFX96 TouchTM/iQ ，Hongshi SLAN-96S/96P,AGS8830, AGS4800