Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative nucleic acid kuzindikira kwa 2019-Novel Coronavirus (2019-nCoV), Fuluwenza A ndi Fuluwenza B nthawi imodzi m'zitsanzo zakupuma kuphatikiza ma oropharyngeal swabs ndi sputum.Ma seti oyambira ndi ma probe olembedwa ndi FAM adapangidwa kuti azitha kuzindikira mitundu yonse ya ORFlab ndi N ya 2019-nCoV, VIC yotchedwa probe ya jini ya RNase P yamunthu ngati chiwongolero chamkati, ROX yolembedwa kuti probe ya M gene of Influenza A, CY5 yolembedwa probe ya NS jini. matenda a Influenza B.
| Zigawo | 50 mayeso / zida |
| RT-PCR reaction buffer | 915 μL × 1 chubu |
| RT-PCR enzyme mix | 75 μL × 1 chubu |
| Kulamulira kwabwino | 100 μL × 1 chubu |
| Kuwongolera kolakwika | 100 μL × 1 chubu |
Kumverera: 200 makope / mL.
Zazikulu: Zachidziwikire: Palibe njira yolumikizirana ndi SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63, Parainfluenza Virus, Human Adenovirus, Human Rhinovirus, Respiratory Syncytial Virus (A), Respiratory Syncytial Virus (B), Enterovirus A, Staphylococcus Aureus, Streptococcus Pneumonia, Klebsiella Pneumonia.
Kulondola: CV ≤ 5%.
Real-Time PCR System:Aehealth Diagenex AL,Molarray MA-6000, ABI 7500, ViiATM7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/Q, Bio-Rad CFX96 TouchTM/iQTM5, SLAN-96S/96P