Chidachi chimagwiritsidwa ntchito mu vitro qualitative kuzindikira jini ya ORF1ab/N/S ya novel coronavirus 2019-nCoV mu zitsanzo za kupuma kuphatikiza oropharyngeal swabs, nasopharyngeal swab, sputum ndi kupanga masinthidwe amtundu wa HV69- 70 del nthawi imodzi.Monga tafotokozera mu database ya GISAID, zopitilira 95% za Omicron zomwe zanenedwa zimaphatikizapo kuchotsedwa mu HV69-70, zomwe zingayambitse kulephera kwa jini ya S (SGTF) pakuyesa kwa PCR.SGTF itha kugwiritsidwa ntchito ngati choyimira choyimira kuti muwonetse Omicron.
Maseti a Primer ndi probe olembedwa ndi FAM adapangidwa kuti azitha kuzindikira jini ya labu ya ORF ya 2019-nCoV, VIC yolembedwa kuti probe ya N gene ya 2019-nCoV, ROX yolembedwa kuti probe ya S gene HV69-70 del mutation ya 2019-nCoV.jini ya RNase P yaumunthu yotengedwa nthawi imodzi ndi chitsanzo choyesa imapereka chiwongolero chamkati kuti chitsimikizire njira yochotsera ma nucleic ndi kukhulupirika kwa reagent.Kafukufuku wolunjika ku jini ya RNase P yamunthu imalembedwa ndi CY5.
Real-Time PCR System: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P, MA 6000;