COVID19-Omicron

Chidachi chimagwiritsidwa ntchito pozindikira zamtundu wa ORF1ab/N/S wa novel coronavirus 2019-nCoV m'zitsanzo zakupuma kuphatikiza ma oropharyngeal swabs, nasopharyngeal swab, sputum ndi kupanga masinthidwe amtundu wa HV69- 70 del nthawi imodzi. Monga momwe zafotokozedwera mu nkhokwe ya GISAID, kupitilira 95% kutsatizana kwa Omicron komwe kunenedwa kumaphatikizapo kuchotsedwa mu HV69-70, komwe kungayambitse kulephera kwa jini ya S (SGTF) pakuyesa kwa PCR. SGTF itha kugwiritsidwa ntchito ngati choyimira choyimira kuti muwonetse Omicron.

Maseti a Primer ndi probe olembedwa ndi FAM adapangidwa kuti azitha kuzindikira zamtundu wa ORF lab 2019-nCoV, VIC yolembedwa probe ya N gene ya 2019-nCoV, ROX yolembedwa probe ya S gene HV69-70 del mutation of 2019-nCoV. jini ya RNase P yaumunthu yotengedwa nthawi imodzi ndi chitsanzo choyesa imapereka chiwongolero chamkati kuti chitsimikizire njira yochotsera ma nucleic ndi kukhulupirika kwa reagent. Kafukufuku wolunjika ku jini ya RNase P yamunthu imalembedwa ndi CY5.