Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative nucleic acid kuzindikira kwa Monkeypox Virus ndi clade yapakati/kumadzulo kwa Africa mu seramu yaumunthu, zitsanzo za lesion exudate ndi nkhanambo.Ma seti oyambira ndi ma probe olembedwa ndi FAM adapangidwa kuti azitha kuzindikira zenizeni za Monkeypox Virus, ROX yolembedwa kuti probe yapakati pa Africa clade, CY5 yolembedwa kuti probe ya West Africa clade.jini ya RNase P yaumunthu yotengedwa nthawi imodzi ndi chitsanzo choyesa imapereka chiwongolero chamkati kuti chitsimikizire njira yochotsera ma nucleic ndi kukhulupirika kwa reagent.Kafukufuku wolunjika ku jini ya RNase P yamunthu imalembedwa ndi VIC.
| Zigawo | 48 Mayeso / zida | 96 Mayeso / zida |
| PCR reaction buffer | 672 μL × 1 chubu | 672 μL × 2 chubu |
| PCR enzyme mix | 50 μL × 1 chubu | 100 μL × 1 chubu |
| Kulamulira kwabwino | 100 μL × 1 chubu | 200 μL × 1 chubu |
| Kuwongolera kolakwika | 100 μL × 1 chubu | 200 μL × 1 chubu |
1. Kumverera: 200 makope/mL.
2. Kudziwikiratu: No cross reaction with Enterovirus(EV), Measles virus(MV), Rubella virus(RV), Varicella-zoster virus(VZV), Dengue virus(DenV), Human Parvovirus B19(HPVB19), Epstein-barr virus (EBv), kachilombo ka herpes 6 (HHV-6)
3. Kulondola: CV ≤ 5%.
Real-Time PCR System: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-Gene TM 6000/Q, Bio-Rad CFX96 TouchTM/iQ TM ，Hongshi SLAN -96S/96P,AGS8830, AGS4800