Mayeso a Rapid COVID-19 Antigen Test ndi colloidal gold immunochromatography yomwe cholinga chake ndi kuzindikira kuti ma antigen a nucleocapsid amachokera ku COVID-19 m'mphuno zamunthu, zokwawa pakhosi kapena malovu kuchokera kwa anthu omwe akuwaganizira kuti ali ndi COVID-19 ndi wothandizira zaumoyo.Ma coronaviruses atsopano ndi amtundu wa β.COVID-19 ndi matenda opatsirana pachimake kupuma.Nthawi zambiri anthu amavutika.Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda;anthu omwe ali ndi matenda asymptomatic amathanso kukhala gwero lopatsirana.Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7.The mawonetseredwe aakulu monga malungo, kutopa ndi youma chifuwa.Kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka nthawi zingapo.Zotsatira ndi zakuzindikiritsa COVID-19 nucleocapsid antigen.Ma antigen nthawi zambiri amawonekera m'mipingo yam'mwamba yopumira kapena m'miyendo yotsika yapanthawi ya matenda.Zotsatira zabwino zimasonyeza kukhalapo kwa tizilombo toyambitsa matenda, koma mgwirizano wachipatala ndi mbiri ya odwala ndi zina zowunikira ndizofunikira kuti mudziwe momwe matendawa alili.Zotsatira zabwino sizimaletsa matenda a bakiteriya kapena kupatsirana ndi ma virus ena.Ma antigen omwe apezeka mwina sangakhale oyambitsa matenda.Zotsatira zoyipa sizimaletsa matenda a COVID-19 ndipo siziyenera kugwiritsidwa ntchito ngati maziko okhawo a chithandizo kapena zisankho zowongolera odwala, kuphatikiza zisankho zowongolera matenda.Zotsatira zoyipa ziyenera kuganiziridwa potengera zomwe wodwalayo adakumana nazo posachedwa, mbiri yakale komanso kupezeka kwazizindikiro zachipatala zomwe zimagwirizana ndi COVID-19 ndikutsimikiziridwa ndi kuyezetsa magazi, ngati kuli kofunikira pakuwongolera odwala.
Reagent iyi imachokera ku colloidal gold immunochromatography assay.Pakuyesa, zotsatsira zachitsanzo zimayikidwa pa Makhadi Oyesera.Ngati pali ma antigen a COVID-19 m'gululi, antigen imamanga ku COVID-19 monoclonal antibody.Pakuthamanga kwa mbali, zovutazo zimasuntha ndi nembanemba ya nitrocellulose kumapeto kwa pepala loyamwa.Mukadutsa mzere woyeserera (mzere T, wokutidwa ndi anti-monoclonal antibody ina ya COVID-19) zovutazo zimagwidwa ndi anti-COVID-19 pamzere woyeserera zikuwonetsa mzere wofiira;podutsa mzere C, mbuzi ya colloidal yagolide yotsutsana ndi akalulu IgG imagwidwa ndi mzere wolamulira (mzere C, wokutidwa ndi kalulu IgG) umasonyeza mzere wofiira.
Zinthu zotsatirazi zikuphatikizidwa mu zida zoyeserera za Rapid COVID-19 Antigen.
Zida Zoperekedwa:
| Mtundu Wachitsanzo | Zipangizo |
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Malovu (okha) |
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Zofunika Koma Zosaperekedwa:
1. Chowerengera nthawi
2. Choyikapo chubu cha zitsanzo
1. Sungani mankhwalawa pa 2-30 ℃, moyo wa alumali ndi miyezi 24 moyesa.
2. Kaseti yoyesera iyenera kugwiritsidwa ntchito mukangotsegula thumba.
3. Ma reagents ndi zida ziyenera kukhala zotentha (15-30 ℃) zikagwiritsidwa ntchito poyesa.
Kutolere Zitsanzo za Throat Swab:
Lolani mutu wa wodwalayo upendekeke pang'ono, pakamwa potseguka, ndikumveka "ah" ndikuwulula matani a pharyngeal mbali zonse.Gwirani swab ndikupukuta matani a pharyngeal kumbali zonse za wodwalayo ndi mphamvu zochepa mmbuyo ndi mtsogolo kwa nthawi yosachepera 3.
Kutolereni Malovu ndi Swab:
Kutoleredwa ndi Maloli Otengera Chida Chotolera Mate:
Zitsanzo Zoyendera ndi Kusungirako:
Zitsanzo ziyenera kuyesedwa mwamsanga mutatha kusonkhanitsa.Ziphuphu kapena chitsanzo cha malovu chikhoza kusungidwa mu Extraction Solution kwa maola 24 pa kutentha kwa firiji kapena 2° mpaka 8°C.Osaundana.
1. Kuyesako kumayenera kuyendetsedwa kutentha (15-30 ° C).
2. Onjezani zitsanzo.
Chitsanzo cha Malovu (kuchokera Chida Chotolera Malovu):
Zabwino
Pali mitundu pamzere C, ndipo mzere wachikuda unawonekera T mzere womwe ndi wopepuka kuposa C mzere, kapena apo
Zoipa
Pali mitundu pamzere C, ndipo mzere wachikuda unawonekera T mzere womwe ndi wakuda kapena wofanana kuposa
Zosalondola
Palibe mitundu pamzere C, monga zikuwonekera pazithunzi zotsatirazi.Mayeso ndi olakwika kapena ndi olakwika
Zolakwika(-): Zotsatira zoyipa ndizongopeka.Zotsatira zoyipa sizimalepheretsa kutenga kachilomboka ndipo siziyenera kugwiritsidwa ntchito ngati maziko okhawo a chithandizo kapena zisankho zina zowongolera odwala, kuphatikiza zisankho zowongolera matenda, makamaka pamaso pa zizindikiro zachipatala zomwe zimagwirizana ndi COVID-19, kapena kwa omwe adadwala. pokhudzana ndi kachilomboka.Ndibwino kuti zotsatirazi zitsimikizidwe ndi njira yoyesera ma molekyulu, ngati kuli kofunikira, kwa Kuwongolera kwa odwala.
Zabwino (+): Zabwino pakukhalapo kwa SARS-CoV-2 antigen.Zotsatira zabwino zimasonyeza kukhalapo kwa tizilombo toyambitsa matenda, koma mgwirizano wachipatala ndi mbiri ya odwala ndi zina zowunikira ndizofunikira kuti mudziwe momwe matenda alili.Zotsatira zabwino sizimaletsa matenda a bakiteriya kapena kugwirizana ndi ma virus ena.Ma antigen omwe apezeka mwina sangakhale chifukwa chotsimikizika cha matenda.
1.Clinical ntchito anawunikidwa ndi zitsanzo mazira, ndi mayeso ntchito akhoza kukhala osiyana ndi zitsanzo zatsopano.
2.Ogwiritsa ntchito ayenera kuyesa zitsanzo mwachangu momwe angathere pambuyo posonkhanitsa.
3.Zotsatira zabwino zoyezetsa sizimaletsa matenda opatsirana ndi tizilombo toyambitsa matenda.
4. Zotsatira zakuyezetsa ma antigen a COVID-19 ziyenera kulumikizidwa ndi mbiri yachipatala, chidziwitso cha miliri, ndi zidziwitso zina zopezeka kwa dokotala yemwe amawunika wodwalayo.
5.Zotsatira zabodza zowonongeka zikhoza kuchitika ngati mlingo wa mavairasi a antigen mu sampuli uli pansi pa malire a mayeso kapena ngati chitsanzocho chinasonkhanitsidwa kapena kutengedwa molakwika;chifukwa chake, zotsatira zoyesa sizichotsa kuthekera kwa matenda a COVID-19.
6.Kuchuluka kwa antigen mu chitsanzo kungachepetse pamene nthawi ya matenda ikuwonjezeka.Zitsanzo zomwe zasonkhanitsidwa pambuyo pa tsiku lachisanu la matenda zimakhala zovuta kwambiri poyerekeza ndi kuyesa kwa RT-PCR.
7.Kulephera kutsatira njira yoyeserera kungasokoneze magwiridwe antchito komanso / kapena kulepheretsa zotsatira zoyeserera.
8. Zomwe zili mu zidazi zikuyenera kugwiritsidwa ntchito pozindikira ma antigen a COVID-19 kuchokera ku zitsanzo za malovu okha.
9. The reagent imatha kuzindikira ma antigen a COVID-19 otheka komanso osatheka. Kuzindikira kumatengera kuchuluka kwa ma antigen ndipo mwina sikungagwirizane ndi njira zina zowunikira zomwe zimachitika pachitsanzo chomwecho.
10. Zotsatira zoyezetsa zosonyeza kuti alibe COVID-19 sizimakhudza matenda ena omwe si a COVID-19.
11. Makhalidwe abwino ndi oipa olosera amadalira kwambiri kuchuluka kwa chiwerengero.Zotsatira zabwino zoyezetsa zimatha kuyimira zotsatira zabodza panthawi yomwe palibe vuto la COVID-19 pomwe matenda achepa.Zotsatira zabodza zoyeserera zimakhala ndi mwayi wochuluka wa matenda obwera chifukwa cha COVID-19.
12. Chipangizochi chidawunikidwa kuti chigwiritsidwe ntchito ndi zitsanzo za anthu okha.
13. Ma antibodies a monoclonal angalephere kuzindikira, kapena kuzindikira mochepa, ma virus a COVID-19 omwe asintha pang'ono amino acid m'chigawo chandamale cha epitope.
14. Kuchita kwa mayeserowa sikunayesedwe kuti agwiritsidwe ntchito kwa odwala opanda zizindikiro ndi zizindikiro za matenda opuma kupuma ndi ntchito zimatha kusiyana ndi anthu asymptomatic.
15. Chidacho chidatsimikiziridwa ndi ma swabs osiyanasiyana.Kugwiritsa ntchito ma swabs amtundu wina kungayambitse zotsatira zabodza.
16. Ogwiritsa ntchito ayenera kuyesa zitsanzo mwachangu momwe zingathere pambuyo posonkhanitsa.
17. Kuwona kwa Mayeso a Rapid COVID-19 Antigen sikunatsimikizidwe kuti kuzindikiridwa/kutsimikizira za kudzipatula kwamtundu wa minofu ndipo sikuyenera kugwiritsidwa ntchito motere.