SARS-CoV-2 (COVID19) ikuchitika padziko lonse lapansi, ndipo katemera amadziwika kuti ndi njira yotsika mtengo komanso yothandiza kwambiri yothanirana ndi mliri wa kachilomboka.Katemera wachikhalidwe nthawi zambiri amagwiritsa ntchito njira zodziwira ma antibody kuti athe kuwunika momwe katemera amagwirira ntchito pogwiritsa ntchito kuyesa kosalowerera ndale;
Njira zachikale zimadya nthawi komanso sizigwira ntchito bwino, nthawi zambiri zimatenga masiku awiri mpaka 4 kuti amalize kuwunika, ndipo chifukwa ambiri a iwo amagwiritsa ntchito ma virus amoyo, Izi ziyenera kuchitidwa mu labotale yachitetezo chachilengedwe 3 kapena pamwamba pa labotale, yomwe ndi nthawi- zowononga ndi zolemetsa, ndipo zimabweretsa zovuta zazikulu pakuwunika kukulitsa ndi kuphatikizira.Chifukwa chake, pakufunika mwachangu njira ina yosavuta komanso yofulumira yomwe ili yoyenera kuwunika ma antibodies oteteza anthu ambiri.
Aehealth COVID19 Neutralization Antibody Quantitative Test Kit imagwiritsidwa ntchito pozindikira kuchuluka kwa ma antibodies a COVID19 mu seramu yamunthu, plasma kapena magazi athunthu.Itha kugwiritsidwa ntchito pozindikira mwachangu, mochulukira komanso tcheru kwambiri mu vitro.Amagwiritsidwa ntchito poyesa kuwunika kothandizira kwa katemera watsopano wa coronavirus ndikuwunika ma antibodies ochepetsa thupi mwa odwala omwe achira atadwala.
Ma antibodies osalowerera ndale amaletsa matendawa poletsa kulumikizana pakati pa kachilombo ka COVID19 ndi ma cell omwe akulandira.Ma antibodies ambiri ochepetsa mphamvu amayankha ku receptor binding domain (RBD) ya protein ya spike, yomwe imamangiriza mwachindunji ku cell surface receptor ACE2.ma antibodies pa intaneti pano akupereka ma antibodies awiri oletsa kutengera CR3022 clone.Ngakhale ma antibodies ambiri omanga a S-protein RBD amapikisana ndi ma antigen omanga ndi ACE2, epitope ya CR3022 simadutsana ndi tsamba lomangirira la ACE2.
Izi sizimalepheretsa kumangidwa kwa ma antibodies.Pomwe CR3022 payokha imangowonetsa zofooka zokha, zawonetsedwa kuti zimalumikizana ndi ma antibodies ena a S-protein RBD kuti achepetse COVID19.
Ntchito yosavuta
- Palibe akatswiri ofunikira kuti aziphunzitsidwa;
- Kufuna kwachitsanzo kochepa, kumangofunika 50 μL;
- Zimagwirizana ndi mitundu ingapo ya zitsanzo: Seramu / Plasma / Magazi Athunthu.
Mkulu tilinazo
- Sensitivity: 98.95%;
- Zofunika: 100%.
Kuchita bwino
- Nthawi yochitira: 15 mins, Nthawi yoyesera: 10s;
- Protable, Lonse ntchito zochitika;
- Batire yomangidwa, yopitilira mayeso a 200 popanda kuyika mphamvu.
Wodalirika
- Kutsimikiziridwa ndi zoyezetsa 3600zachipatala, zoyezetsa 1500 ndi zitsanzo za munthu yemwe ali ndi kachilombo, zoyesa 900 za oponya katemera, 1200 zoyesedwa ndi anthu wamba.;
- Deta yachipatala imapezedwa kwa oteteza ndi katemera wosagwiritsidwa ntchito, katemera wa nucleic acid, katemera wa mapuloteni, ndi anthu omwe ali ndi kachilombo, anthu abwinobwino.
- Kuchepetsa mtengo woletsa mtengo wa 30%.
Mfundo yofunikira yomwe imagwiritsidwa ntchito pagululi ndi mpikisano wa immunochromatographic. Mzere wozindikira (T-line) pa mzere woyesera umakutidwa ndi angiotensin converting enzyme 2 ndipo mzere wowongolera (C-line) wokutidwa ndi anti-kalulu IgG antibody. conjugate pad idakutidwa ndi mapuloteni otchedwa COVID19 RBD omwe amadziwika kuti COVID19 RBD komanso antibody ya IgG yotchedwa fluorescent.Panthawi yodziwika, chitsanzocho chikakhala ndi antibody yoyesedwa, conjugate ya chinthu choyesera mu chitsanzo ndi antigen ya fluorescent imapanga chitetezo chamthupi ndipo chitetezo cha mthupi sichingathenso kumangirira ku angiotensin-converting enzyme 2 osasunthika pa nembanemba ya nitrocellulose. .Antigen conjugate ya fluorescent yomwe siimangika ku antibody kuti iyesedwe idzamangiriza ku angiotensin converting enzyme 2 yosasunthika pa nembanemba ya nitrocellulose kuti ipange chingwe chodziwikiratu (T).
- Kuyeza mayeso pamaso katemera;
- Kuyang'anira zotsatira pambuyo katemera;
- Kuwunika kwachiwopsezo cha matenda achiwiri a anthu omwe ali ndi kachilombo;
- Kuunika kwachiwopsezo cha kuthekera kwa anthu abwinobwino (kuphatikiza matenda opanda zizindikiro) kutenga matenda;
- Kuyesa kukana ma virus.